Journal: Pharmaceuticals

Article Title: New Genetic Bomb Trigger: Design, Synthesis, Molecular Dynamics Simulation, and Biological Evaluation of Novel BIBR1532-Related Analogs Targeting Telomerase against Non-Small Cell Lung Cancer

doi: 10.3390/ph15040481

Figure Lengend Snippet: Telomerase activity in cancer cells as a percent of control cells with target compounds 29a , 36b , and 39b .

Article Snippet: The human A549 (epithelial cell lung carcinoma, ATCC, Manassas, VA, USA), HCC44 (non-small cell lung adenocarcinoma, Leibniz Institute DSMZ-German Collection of the Microorganisms and the Cell Cultures, Braunschweig, Germany), and NCI-H23 (non-small cell lung adenocarcinoma, ATCC, Manassas, VA, USA) cell lines (compounds 29a , 36b , and 39b ) were diluted to 10 μM and incubated for 48 h before being tested using the TRAP method.

Techniques: Activity Assay, Control

Journal: medRxiv

Article Title: Potent clinical prognostic and systemic adjuvant therapeutic value of plasma fractalkine in PD-L1/PD-1 blockade immunotherapy for lung cancer

doi: 10.1101/2022.06.16.22276511

Figure Lengend Snippet: (a) The bar graphs show plasma concentrations of the selected proteins in responders (R, n=17, 26), progressors (PR, n=38, 59), hyperprogressors (HPR, n=3), patients with stable disease (SD, n=5, 11) and age-matched healthy donors (H, n=4, 6) before (baseline, B) and after the first cycle of immunotherapy (1C). Error bars are shown (standard deviations, SD), and relevant statistical comparisons by Mann-Whitney U tests are shown within the graphs. (b) Correlation between FKN plasma concentration and diversity index with Spearmańs test. (c) ROC analysis of FKN concentration as a predictor of objective responses. The calculated cut-off value for the statistics provided in the graph is shown. (d) Kaplan-Meier plot of PFS stratifying the patients according to the FKN cut-off value identified in the ROC curve (N=42). The associated P value is shown. (e) As in (d) but plotting OS. n= 46 (17 above the cut-off, 29 below). (f) Kaplan-Meier survival plot of lung adenocarcinoma (LUAD) patients. Patients were stratified according to FKN transcriptional expression level within tumor samples. Data were retrieved from the TCGA database and exported with TIMER2.0. Differences in survival were evaluated with the Log rank test, and associated P values are shown within the graph. n=515. HR=0.827; 95% confidence interval=0.784 to 0.945. (g) As in (f) but plotting squamous lung carcinoma (LUSC) patients n=501. HR=1.03; 95% confidence interval=0.885 to 1.095. (h) Real time cell growth (RTCA) of 3LL cell lines engineered to secrete the indicated cytokines. Relevant statistical comparisons of delta-cell indexes after 50 h of culture were carried out by ANOVA. (i) Bar graphs with cell growth rates for the indicated 3LL cell lines relative to the growth of unmodified 3LL cells. Error bars are shown (SD). Statistical comparisons by ANOVA and Tukeýs pairwise comparison tests are indicated. *, **, ***, indicate significant (p<0.05), very significant (p<0.01) and highly significant (p<0.001) differences; ns, non-significant differences.

Article Snippet: FKN concentration was quantified in a collection of 5 human lung adenocarcinoma cell lines (Calu6, H23, A549, HCC44, H358) and 5 human lung squamous carcinoma cell lines (H520, H1703, H226, H2170, SW900) from the American Type Culture Collection.

Techniques: Clinical Proteomics, MANN-WHITNEY, Concentration Assay, Expressing, Comparison

Journal: medRxiv

Article Title: Potent clinical prognostic and systemic adjuvant therapeutic value of plasma fractalkine in PD-L1/PD-1 blockade immunotherapy for lung cancer

doi: 10.1101/2022.06.16.22276511

Figure Lengend Snippet: (a) Evaluation of tumor infiltration with the indicated selected immune cell populations, and correlation analyses of FKN transcriptional expression and immune infiltrates from the TCGA database. Analyses were restricted to lung adenocarcinoma samples (n=515). Spearman correlation with different immune populations were identified by several algorithms (CIBERSORT, quanTIseq, xCell, TIDE) and an adjustment based on tumor purity was employed to minimize the potential interaction of low tumor cell quantities. Each dot represents a single tumor sample. Spearman’s rho value and p values are provided within the graphs. (b) Kaplan-Meier plot of OS according to FKN expression in a lung adenocarcinoma cohort registered in the TCGA database (n=240). Patients were stratified according to the median FKN expression value. Survival differences were tested by the Log rank test. Dotted lines represent 95% confidence intervals. (c) Correlation between tumor FKN and PD-L1 transcriptional expression by Spearmańs test. Relevant statistical results are presented within the graphs. (d) As in (c) but plotting PD-1 transcriptional expression levels.

Article Snippet: FKN concentration was quantified in a collection of 5 human lung adenocarcinoma cell lines (Calu6, H23, A549, HCC44, H358) and 5 human lung squamous carcinoma cell lines (H520, H1703, H226, H2170, SW900) from the American Type Culture Collection.

Techniques: Expressing